Tag: spillover
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Bridging Gaps in Dengue Surveillance: Addressing Sylvatic DENV-2 Misclassification in RT-qPCR Assays
Introduction: The surveillance blind spot in dengue diagnostics Dengue viruses are routinely monitored through molecular serotyping assays that identify circulating serotypes in patient samples. Recent work by Suppiah and colleagues highlights a significant blind spot: sylvatic dengue virus type 2 (DENV-2) strains are increasingly misclassified as “Undetermined Serotype” when using common commercial RT-qPCR serotyping assays.…