Tag: spheroids
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How 3D Culture Environments Shape Spheroid Morphology, Drug Response, and Invasion in Pancreatic Cancer Cells
Introduction Pancreatic cancer remains a formidable challenge, with 3D cell culture offering a more physiologically relevant model than traditional 2D systems. This study compares how two common scaffold-free 3D platforms— Poly(2-hydroxyethyl methacrylate) (Poly-HEMA or PH) coated plates and ultra-low attachment (ULA) plates—influence spheroid formation, drug sensitivity to gemcitabine, and invasion dynamics in three pancreatic cancer…
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3D Culture Environments Shape Pancreatic Cancer Spheroids
Introduction Three-dimensional (3D) cell culture models are increasingly used to study pancreatic cancer (PCa) because they better mimic the tumor microenvironment than traditional 2D systems. This article reviews how two commonly used scaffold-free 3D platforms—poly(2-hydroxyethyl methacrylate) (PH)-coated plates and ultra-low attachment (ULA) plates—shape spheroid morphology, drug sensitivity, and invasion across PCa cell lines. By comparing…
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Platform-Driven Spheroid Dynamics: How 3D Culture Environments Shape Pancreatic Cancer Behavior
Introduction: Why the Culture Environment Matters in Pancreatic Cancer Research Pancreatic ductal adenocarcinoma (PDAC) remains one of the most lethal cancers, partly due to its complex microenvironment and resistance to chemotherapy. Traditional 2D cultures fail to capture the 3D architecture and mechanical cues that influence tumor cell behavior. In this study, researchers compared two common…