Tag: PacBio HiFi
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Pangenome-Informed Long-Read Amplicons Enable High-Resolution Wheat Microbiome Profiling
Introduction: A new era of microbiome profiling in crops Understanding the microbial communities living on wheat leaves is crucial for crop health and yield. Traditional short-read ribosomal barcodes often miss fine-scale diversity within dominant phyllosphere taxa. A recent approach leverages pangenome-informed, taxon-specific long-read amplicons to achieve species- and strand-level resolution in real agricultural settings. Here,…
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High-Resolution Plant Microbiome Profiling with Pangenome-Informed Long-Read Amplicons in Wheat Leaves
Introduction: elevating microbiome profiling in the wheat phyllosphere Understanding the complex microbial communities on wheat leaves is essential for disease management and crop health. Traditional ribosomal barcodes (16S/ITS) offer broad taxonomic coverage but often fall short in resolving closely related strains. A recently developed approach uses pangenome-informed, taxon-specific long-read amplicons to achieve species- and strain-level…