Overview of a long-term molecular survey in protected catchments Maintaining safe drinking water from natural catchments requires understanding protozoan pathogens in wildlife. This longitudinal study analyzes Cryptosporidium and Giardia from 8,695 fecal DNA samples collected across 16 years (2009–2024) in Melbourne, Australia’s protected catchments. Using PCR-based sequencing and phylogenetic analyses, researchers identified 12 Cryptosporidium species/genotypes…