Tag: 3D cell culture
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How 3D Culture Environments Shape Spheroid Morphology, Drug Response, and Invasion in Pancreatic Cancer Cells
Introduction Pancreatic cancer remains a formidable challenge, with 3D cell culture offering a more physiologically relevant model than traditional 2D systems. This study compares how two common scaffold-free 3D platforms— Poly(2-hydroxyethyl methacrylate) (Poly-HEMA or PH) coated plates and ultra-low attachment (ULA) plates—influence spheroid formation, drug sensitivity to gemcitabine, and invasion dynamics in three pancreatic cancer…
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3D Culture Environments Shape Pancreatic Cancer Spheroids
Introduction Three-dimensional (3D) cell culture models are increasingly used to study pancreatic cancer (PCa) because they better mimic the tumor microenvironment than traditional 2D systems. This article reviews how two commonly used scaffold-free 3D platforms—poly(2-hydroxyethyl methacrylate) (PH)-coated plates and ultra-low attachment (ULA) plates—shape spheroid morphology, drug sensitivity, and invasion across PCa cell lines. By comparing…